Haplotype diversity in mitochondrial DNA hypervariable regions I and II in Maracaibo population (Venezuela).

Abstract: The control region of the human mitochondrial DNA (mtDNA) is highly polymorphic due to a rapid rate of evolution. The hypervariable segments HVI and HVII of the mitochondrial control region (D-loop) are the most variable part of human mitochondrial DNA. Blood samples from 50 unrelated individuals born from Maracaibo City (Venezuela) were obtained from routine paternity. Polymerase chain reaction amplification products were purified and fluorescent-based capillary electrophoresis sequencing method, the sequences were analysed and it is compared DNA sequences with the rCRS. The dominant haplogroups corresponded to Amerindians followed by African. The nucleotide positions most common for HVII was 263G (100%), 315.1C (100%), 73G (94%), and 309.1C (66%) and for HVI 16223T (74%), 16362C (56%), 16319A (40%) and 16111T (34%). [Copyright &y& Elsevier]