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Effect of Rapamycin on TGF-β1-induced epithelial-mesenchymal transition in LoVo colonic adenocarcinoma cells.
- Source :
- Journal of Nanjing Medical University; Jan2009, Vol. 23 Issue 1, p15-19, 5p
- Publication Year :
- 2009
-
Abstract
- Abstract: Objective: To investigate the effect of Rapamycin on epithelial-mesenchymal transition(EMT) of LoVo colonic adenocarcinoma cells in vitro. Methods: Cultured LoVo colonic adenocarcinoma cells were divided into three groups: negative control group, EMT-inducing group(TGF-β<subscript>1</subscript>) and EMT-interfering group(TGF-β<subscript>1</subscript> plus Rapamycin). E-cadherin expression in LoVo cells was detected by Western Blot, while the expression of vimentin was evaluated through immunocytochemistry. The Snail mRNA in LoVo cells was examined by RT-PCR. Results: TGF-β<subscript>1</subscript> induced LoVo cell switching from polygonal to spindle-shaped. TGF-β<subscript>1</subscript> enhanced the expression of vimentin, but lowered the level of E-cadherin. In contrast, Rapamycin impaired the transition induced by TGF-β<subscript>1</subscript>. Rapamycin dramatically abrogated TGF-β<subscript>1</subscript>-induced vimentin expression and restored E-cadherin expression in LoVo cells. Rapamycin significantly repressed the up-regulation of Snail mRNA expression induced by TGF-β<subscript>1</subscript>. Conclusion: Rapamycin dramatically abrogated TGF-β<subscript>1</subscript> induced Snail mRNA expression in LoVo cells, hence inhibiting EMT of these cells in vitro. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 10074376
- Volume :
- 23
- Issue :
- 1
- Database :
- Supplemental Index
- Journal :
- Journal of Nanjing Medical University
- Publication Type :
- Academic Journal
- Accession number :
- 43312519
- Full Text :
- https://doi.org/10.1016/S1007-4376(09)60020-4