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Activation of Endothelial Cells in Conduit Veins of Dogs With Heart Failure and Veins of Normal Dogs After Vascular Stretch by Acute Volume Loading.
- Source :
- Journal of Cardiac Failure; Jun2009, Vol. 15 Issue 5, p457-463, 7p
- Publication Year :
- 2009
-
Abstract
- Abstract: Background: The venous endothelium is a key regulator of central blood volume, organ perfusion, and hemostasis in heart failure (HF). We previously reported activation of the inflammatory/oxidative program in venous endothelial cells collected from decompensated HF patients. The underlying causes are unknown. We tested the hypothesis that the pro-inflammatory state of HF and vascular strain associated with congestion can activate the endothelial inflammatory/oxidative and hemostatic programs. Methods and Results: We studied 6 normal (NL) dogs (left ventricular ejection fraction [LVEF] >50%, central venous pressure [CVP] = 8 ± 2 mm Hg) and 6 dogs with HF (LVEF ∼30%, CVP 8 ± 2 mm Hg) produced by intracoronary microembolizations. Normal dogs were studied at baseline and 1 hour after fluid load to a target CVP ≥20 mm Hg. Endothelial cells were scraped from jugular veins; mRNA expression was analyzed by reverse transcription polymerase chain reaction. The endothelial inflammatory/oxidative and hemostatic programs were significantly activated in HF dogs compared with NL. In NL dogs, fluid load significantly activated the endothelial inflammatory/oxidative and hemostatic programs, and, concurrently, caused a significant increase in plasma neurohumoral indices to levels that approached those of HF dogs. Conclusions: The pro-inflammatory state of HF and vascular strain associated with congestion can both activate venous endothelial cells in dogs in a manner consistent with that seen in HF patients. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 10719164
- Volume :
- 15
- Issue :
- 5
- Database :
- Supplemental Index
- Journal :
- Journal of Cardiac Failure
- Publication Type :
- Academic Journal
- Accession number :
- 40629933
- Full Text :
- https://doi.org/10.1016/j.cardfail.2008.12.006