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IN VIVO STUDIES OF CARDIOPROTECTION INDUCED BY VOLATILE ANAESTHETICS -- EXPERIMENTAL MODEL IN RODENTS.
- Source :
- Bulletin of the University of Agricultural Sciences & Veterinary Medicine Cluj-Napoca. Veterinary Medicine; 2008, Vol. 65 Issue 1, p406-411, 6p, 1 Diagram, 2 Graphs
- Publication Year :
- 2008
-
Abstract
- Myocardial ischemic/reperfusion (I/R) injury remains a major public health burden and cardioprotection defined as the totality of both non-pharmacological and pharmacological interventions aimed at preventing the reperfusion injury, still represents a stringent necessity. There is accumulating evidence that volatile anaesthetics induce myocardial preconditioning when given prior to ischemia and they also protect the heart at the very onset of reperfusion, an effect referred to as anaesthetic postconditioning. The aims of the present study were: (i) to develop and validate an experimental model of regional myocardial I/R injury in rat hearts in situ and (ii) to investigate in this model the protective effects of cardioprotection elicited by anesthetic pre- and postconditioning with Isoflurane and Sevoflurane. METHODS: Anaesthetized rats (n = 6-8/group) subjected to 30 min ischemia by coronary artery ligation and 2 h reperfusion were randomly assigned to one of the following groups: 1) Control group (Ctrl, no other intervention); 2) Ischemic preconditioning (I-PreC, preconditioning by three episodes of 5 min ischemia interspersed with three episodes of 10 min reperfusion); 3) Isoflurane preconditioning (Iso-PreC, preconditioning by three episodes of 5 min isoflurane 2.1% interspersed with three 10 min washout episodes); 4) Isoflurane postconditioning (Iso-PostC, 5 min postconditioning with isoflurane 2.1% started 3 min prior to and administered for 2 min within reperfusion); 5) Sevoflurane preconditioning (Sevo-PreC, preconditioning by two episodes of 5 min sevoflurane 2.5% interspersed with two 10 min washout episodes) and 6) Sevoflurane postconditioning (Sevo-PostC, 5 mitt postconditioning with sevoflurane 2.5% started 3 min prior to and administered for 2 min within reperfusion). RESULTS: Myocardial injury expressed as the percent of infarct to risk area ratio (I/R%) was significantly reduced in I-PreC (14.3 ± 6.8%) and Iso-PreC (17 ± 5.1%) and Sevo-PreC (19.5±8.5%) when compared to the Ctrl group (46.4±6.5%, mean ± SD; P<0.01), but no protection was found with the postconditioning protocols. CONCLUSIONS: We have validated an experimental model of regional ischemia that can be used in studies of in vivo cardioprotection. In this model anesthetic preconditioning but not postconditioning was associated with infarct size reduction. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 18435270
- Volume :
- 65
- Issue :
- 1
- Database :
- Supplemental Index
- Journal :
- Bulletin of the University of Agricultural Sciences & Veterinary Medicine Cluj-Napoca. Veterinary Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 36530547