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Optimized two-dimensional thin layer chromatography to monitor the intracellular concentration of acetyl phosphate and other small phosphorylated molecules.
- Source :
- Biological Procedures Online; 2008, Vol. 10 Issue 1, p36-46, 11p, 3 Diagrams, 5 Graphs
- Publication Year :
- 2008
-
Abstract
- The article discusses a study that provided an in-depth methods supplement to optimized 2D-TLC protocol, which can be used to monitor acetyle phosphate (acetyl-P) and other small, phosphorylated compounds. It is noted that all bacterial strains used in this study are derivatives of E.coli K-12. The study concluded that the protocol results in greatly improved reproducibility and allows precise measurements of the intracellular concentration of acetyl-P, as well as that of other small phosphorylated molecules.
- Subjects :
- PHOSPHORUS compounds
PHOSPHATES
BIOCHEMISTRY
BIOLOGY
BIOTECHNOLOGY
Subjects
Details
- Language :
- English
- ISSN :
- 14809222
- Volume :
- 10
- Issue :
- 1
- Database :
- Supplemental Index
- Journal :
- Biological Procedures Online
- Publication Type :
- Academic Journal
- Accession number :
- 36171645
- Full Text :
- https://doi.org/10.1251/bpo141