Optimized two-dimensional thin layer chromatography to monitor the intracellular concentration of acetyl phosphate and other small phosphorylated molecules.

The article discusses a study that provided an in-depth methods supplement to optimized 2D-TLC protocol, which can be used to monitor acetyle phosphate (acetyl-P) and other small, phosphorylated compounds. It is noted that all bacterial strains used in this study are derivatives of E.coli K-12. The study concluded that the protocol results in greatly improved reproducibility and allows precise measurements of the intracellular concentration of acetyl-P, as well as that of other small phosphorylated molecules.