Microarray validation: factors influencing correlation between oligonucleotide microarrays and real-time PCR.

The article discusses a study which compiled data from five independent experiments to establish the degree of correlation between two-color inkjet printed 60-mer oligonucleotide microarrays and quantitative real-time polymerase chain reaction (qPCR ) using SYBR green. Using the compiled data set, the study aims to identify factors that influence the correlation between these two techniques. The effects of several parameters on the correlation of data were also investigated.