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Ulcerative Colitis Is a Disease of Accelerated Colon Aging: Evidence From Telomere Attrition and DNA Damage.
- Source :
- Gastroenterology (00165085); Aug2008, Vol. 135 Issue 2, p410-418, 9p
- Publication Year :
- 2008
-
Abstract
- Background & Aims: Telomere shortening is implicated in cancer and aging and might link these 2 biologic events. We explored this hypothesis in ulcerative colitis (UC), a chronic inflammatory disease that predisposes to colorectal cancer and in which shorter telomeres have been associated with chromosomal instability and tumor progression. Methods: Telomere length was measured by quantitative polymerase chain reaction in colonocytes and leukocytes of 2 different sets of UC patients and compared with normal controls across a wide range of ages. For a subset of patients, telomere length was measured in epithelium and stroma of right and left colon biopsy specimens. A third set of biopsy specimens was analyzed for phosphorylation of histone H2AX (γH2AX), a DNA damage signal, by immunofluorescence and for telomere length by quantitative fluorescence in situ hybridization. Relationships between telomere length, γH2AX intensity, age, disease duration, and age of disease onset were explored. Results: Colonocyte telomeres shorten with age almost twice as rapidly in UC patients as in normal controls. This extensive shortening occurs within approximately 8 years of disease duration. Leukocyte telomeres are slightly shorter in UC patients than in controls, but telomeres of colon stromal cells are unaffected. γH2AX intensity is higher in colonocytes of UC patients than in controls and is not dependent on age or telomere length. Conclusions: Colonocytes of UC patients show premature shortening of telomeres, which might explain the increased and earlier risk of cancer in this disease. Shorter leukocyte telomeres and increased γH2AX in colonocytes might reflect oxidative damage secondary to inflammation. [Copyright &y& Elsevier]
- Subjects :
- ULCERATIVE colitis
DNA damage
TELOMERES
LEUCOCYTES
Subjects
Details
- Language :
- English
- ISSN :
- 00165085
- Volume :
- 135
- Issue :
- 2
- Database :
- Supplemental Index
- Journal :
- Gastroenterology (00165085)
- Publication Type :
- Academic Journal
- Accession number :
- 33836199
- Full Text :
- https://doi.org/10.1053/j.gastro.2008.04.008