Expression and Purification of Histidine-Tagged Transcription Factors.

The analysis of transcription factors in terms of their structure, function, and mode of interaction with DNA and other components of the transcriptional machinery has been greatly facilitated in recent years by the identification of genes encoding these proteins. Not only has this allowed elucidation of primary sequence and structure, and identification of transcription factor subfamilies and functional domains, but it has also permitted synthesis of large quantities of recombinant proteins for use in DNA binding, protein-protein interaction, and in vitro transcription assays. [ABSTRACT FROM AUTHOR]