Analysis of Protein Interactions Between Protein Phosphatase 1 and Noncatalytic Subunits Using the Yeast Two-Hybrid Assay.

A large variety of methods exist to identify protein-protein interactions. The two hybrid method is unique in that interactions are assayed in vivo. The method can be used "analytically" or "preparatively." In the analytical application, one surveys interactions between two proteins known to interact by comparing interaction of mutant proteins. The preparative two hybrid method allows one to identify putative proteins that interact with a given protein (called the "bait") by screening libraries. This chapter will focus only on the analytical application of the two hybrid assay to analyze interactions of mutant protein phosphatase 1 (PP1) molecules with some PP1 noncatalytic subunits. For examples of the latter screening method, readers are referred to original papers that employed the method to isolate PP1 interacting proteins (1-3). [ABSTRACT FROM AUTHOR]