Purification of Tyrosine-Phosphorylated Proteins by Immunoaffinity Chromatography and Direct Cloning of Their cDNAs from Bacterial Expression Libraries.

Growth factor receptors endowed with intrinsic tyrosine-kinase activity (receptor tyrosine kinases, RTKs) are capable of intracellular signal transduction through their ability to autophosphorylate and to phosphorylate cellular proteins, globally referred to as substrates (refs. 1-3 and references therein). [ABSTRACT FROM AUTHOR]