Influenza Vaccines - Challenges in Mammalian Cell Culture Technology.

Experimental data as well as simulation results obtained by mathematical models for an influenza vaccine process with adherent MDCK cells clearly show that total cell number, specific virus replication rate and cell death due to virus infection (apoptosis) are the main factors to be taken into account for achieving high virus yields. In contrast, supply of cellular precursors and ATP for the synthesis of viral genome and virus specific proteins as well as multiplicity of infection seems not to limit virion formation. [ABSTRACT FROM AUTHOR]