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Isolation of Plasmids from E. coli by Boiling Lysis.
- Source :
- E. coli Plasmid Vectors; 2003, p79-82, 4p
- Publication Year :
- 2003
-
Abstract
- The boiling lysis procedure (1) is quick to perform and, therefore, especially suitable for screening large numbers of small-volume Escherichia coli cultures. It is described with different adaptations in a variety of protocol books (2,3). The quality of the isolated plasmid DNA is lower than that from an alkaline lysis miniprep, but it is sufficient for restriction analysis. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISBNs :
- 9781588291516
- Database :
- Supplemental Index
- Journal :
- E. coli Plasmid Vectors
- Publication Type :
- Book
- Accession number :
- 33149441
- Full Text :
- https://doi.org/10.1385/1-59259-409-3:79