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Phage Display Technology.

Authors :
Walker, John M.
George, Andrew J. T.
Urch, Catherine E.
Johns, Michael
Palmer, Donald B.
Source :
Diagnostic & Therapeutic Antibodies; 2000, p461-470, 10p
Publication Year :
2000

Abstract

Over the past few years, considerable effort has gone into genetic engineering and manipulation of antibody molecules. One consequence of this research has been the use of filamentous phage as a vehicle on which to display antibody fragments. It is possible to express a variety of molecules on the surface of phage, including peptides and antibody fragments. With regard to antibodies, libraries of both sFv and Fab fragments have been successfully cloned onto the surface of bacteriophages, usually via the N terminal of the gene III product, although expression on the gene VIII product has also been employed to isolate low-affinity antibodies. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISBNs :
9780896037984
Database :
Supplemental Index
Journal :
Diagnostic & Therapeutic Antibodies
Publication Type :
Book
Accession number :
33149078
Full Text :
https://doi.org/10.1385/1-59259-076-4:461