Rapid inhibition of Ca2+ influx by neurosteroids in murine embryonic sensory neurones.

Abstract: The non-genomic role of neuroactive steroids on [Ca²⁺]_i transients induced by GABA receptor activation was investigated in cultured dorsal root ganglia (DRG) neurones at embryonic stage E13. [Ca²⁺]_i measurements were performed with Fura-2 fast fluorescence microfluorimetry. Application of the GABA_A receptor agonist muscimol (Musci) evoked an increase in [Ca²⁺]_i, confirming the excitatory effect of GABA at this embryonic stage. The muscimol-induced [Ca²⁺]_i response was inhibited by progesterone (Proges) and its primary metabolite allopregnanolone (Allo) in a rapid, reversible and dose-dependent manner. These calcium transients were suppressed in the absence of external Ca²⁺ or in the presence of Ni²⁺ +Cd²⁺ suggesting an involvement of voltage-activated Ca²⁺ channels. In contrast, none of these steroids affected the resting [Ca²⁺]_i nor exhibited any inhibitory effect on 50mM KCl-induced [Ca²⁺]_i increases. In view of the well-established potentiation of GABA_A receptor by direct binding of neurosteroids, the inhibitory effects described in this study seem to involve distinct mechanisms. This new inhibitory effect of progesterone is observed at low and physiological concentrations, is rapid and independent of RU38486, an antagonist of the classic progesterone receptor, probably involving a membrane receptor. Using RT-PCR, we demonstrated the expression of progesterone receptor membrane component 1 (Pgrmc1), encoding 25-Dx, a membrane-associated progesterone binding protein in DRG neurones at different stages of development. In conclusion, we describe for the first time a rapid effect of progestins on embryonic DRG neurones involving an antagonistic effect of progesterone and allopregnanolone on GABA_A receptors. [Copyright &y& Elsevier]