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Blood storage at 4°C—factors involved in DNA yield and quality.

Authors :
Richardson, Andrea J.
Narendran, Niro
Guymer, Robyn H.
Vu, Hien
Baird, Paul N.
Source :
Journal of Laboratory & Clinical Medicine; Jun2006, Vol. 147 Issue 6, p290-294, 5p
Publication Year :
2006

Abstract

Background: Whole blood provides one of the most common sources of both high-quality DNA and high-quantity DNA for molecular biological purposes. Typically, blood storage at 4°C is short term, which ranges from a few days to a few weeks. However, long-term storage usually involves blood being frozen, with a resultant loss in DNA yield. The authors examined the effects of long-term storage at 4°C. Methods: Duplicate blood samples were collected from 301 participants (aged 20–98 years) enrolled as part of ongoing studies. Samples were stored at 4°C for between 11 days and 922 days, and DNA was subsequently extracted using a phenol/chloroform procedure. Results: A negative correlation of the number of storage days existed at 4°C with DNA yield. The main determinant on DNA yield was the age of the participant in the study, with older persons having a lower DNA yield. Conclusions: Long-term storage of blood at 4°C does have a detrimental effect on DNA yield, but this effect seemed less significant than the age of a person. The impact of age of a person or storage time has a minimal impact on DNA quality. Therefore, storage of blood at 4°C offers an acceptable alternative to frozen storage. [Copyright &y& Elsevier]

Details

Language :
English
ISSN :
00222143
Volume :
147
Issue :
6
Database :
Supplemental Index
Journal :
Journal of Laboratory & Clinical Medicine
Publication Type :
Academic Journal
Accession number :
21623697
Full Text :
https://doi.org/10.1016/j.lab.2006.01.005