Data on Biophysics and Physicobiology Described by Researchers at Graduate School of Science (Rapid in vitro method to assemble and transfer DNA fragments into the JCVI-syn3B minimal synthetic bacterial genome through Cre/loxP system).

Researchers at the Graduate School of Science in Osaka, Japan, have developed a rapid in vitro method to assemble and transfer DNA fragments into the JCVI-syn3B minimal synthetic bacterial genome through the Cre/loxP system. This method aims to overcome challenges such as E. coli growth inhibition and unexpected recombination, especially with AT-rich DNA sequences. The study successfully obtained recombinant colonies in a shorter period without recombination issues, making it potentially applicable to other gene manipulation systems based on the Cre/loxP system. For more information, readers can access the full article in Biophysics and Physicobiology. [Extracted from the article]