Coupling CRISPR Scanning with Targeted Chromatin Accessibility Profiling using a Double-Stranded DNA Deaminase.

The article discusses a new method called targeted deaminase-accessible chromatin sequencing (TDAC-seq) that combines CRISPR scanning with chromatin accessibility profiling to study gene regulation and develop gene therapies. By using a double-stranded DNA deaminase, researchers can analyze chromatin accessibility at specific loci with high depth and resolution, allowing for the functional dissection of cis-regulatory elements. The study demonstrates the application of TDAC-seq in activating fetal hemoglobin in human hematopoietic stem cells and in screening enhancers controlling the globin locus. This innovative approach enables high-resolution mapping of chromatin fibers through genome editing techniques. [Extracted from the article]