New Life Science Study Findings Have Been Reported by Investigators at Westlake University (Robust and Irreversible Sortase-mediated Ligation By Empolyment of Sarkosyl).

A recent study conducted at Westlake University in Zhejiang, China, has introduced a new method called sarkosyl-enhanced Sortase-mediated ligation (SML) to address limitations in peptide and protein ligation techniques. By utilizing sarkosyl, a detergent, this method enhances substrate solubility, sorting motif accessibility, and renders the reaction irreversible, resulting in excellent ligation yields. The research findings suggest that this approach could be widely applied in peptide and protein chemistry, particularly for conjugating aggregation-prone substrates, and offer insights into detergent-driven equilibrium mechanisms. The study has been peer-reviewed and published in Chemistry - A European Journal. [Extracted from the article]