Effects of biodegradable microplastics on the crustacean isopod Idotea balthica basteri Audouin, 1826.

Plastic pollution is a notable environmental issue, being plastic widespread and characterized by long lifetime. Serious environmental problems are caused by the improper management of plastic end-of-life. In fact, plastic litter is currently detected in any environment. Biodegradable Polymers (BPs) are promising materials if correctly applied and managed at their end of life, to minimize environmental problems. However, poor data on the fate and toxicity of BPs on marine organisms still limit their applicability. In this work we tested the effects of five biodegradable polymers (polybutylene succinate, PBS; polybutylene succinate-co-butylene adipate, PBSA; polycaprolactone, PCL; poly (3-hydroxybutyrates, PHB; polylactic acid, PLA) widely used for several purposes. Adult individuals of the isopod Idotea balthica basteri were fed on these polymers for twenty-seven days by adding biodegradable microplastic polymers (BMPs) to formulated feeds at two concentrations, viz. 0.84 and 8.4 g/kg feed. The plastic fragments affected the mortality rates of the isopods, as well as the expression levels of eighteen genes (tested by Real Time qPCR) involved in stress response and detoxification processes. Our findings confirmed that I. balthica basteri is a convenient model organism to study the response to environmental pollution and emerging contaminants in the aquatic environment, and highlighted the need for the correct use of BMPs. [Display omitted] • Mortality of marine isopods is observed upon ingestion of PCL and PBSA at ambient concentrations. • Exposures to PCL and PBSA at 0.84 g/kg induce down-regulation of 14 and 13 genes, respectively. • Exposures to PLA and PBSA at 8.4 g/kg induce up-regulation of 13 and 14 genes, respectively. • The survival rate of marine invertebrates is influenced by the size of MPs ingested. [ABSTRACT FROM AUTHOR]