Musa basjoo Sieb polysaccharide improves inflammation in RAW264.7 cells and zebrafish colitis.

Musa basjoo Sieb polysaccharide (MBSP) is the active ingredient of Musa basjoo Sieb. The purpose of this study was to preliminarily characterize the structural properties of MBSP, and to explore its ameliorating effect LPS-induced macrophage RAW264.7 inflammation and its therapeutic effect on DSS-induced zebrafish colitis. The data indicated that the average molecular weight of MBSP was 393.4 kDa, which was an acidic heteropolysaccharide composed of seven monosaccharides. In the LPS-induced macrophage RAW264.7 inflammation model, we found that MBSP significantly reduced the level of inflammatory mediators (p < 0.001). The release of NO, IL-6, and TNF-α was reduced by 48.21%, 65.45%, and 86.49%, respectively. And MBSP modulated intracellular ROS levels. This might be related to the activation of NF-κB/MAPK signaling pathway by MBSP. Additionally, MBSP significantly improved the oxidative stress status of UC zebrafish. SOD, CAT, GSH-Px were increased by 50.58%, 39.07% and 65.86%, and MDA, MPO were decreased by 26.00% and 50.98%, respectively (p < 0.001). Meanwhile, MBSP was also found to be effective in improving UC by activating the TLR4/NF-κB/MAPK signaling pathway in vivo. In conclusion, the present study characterized the structural properties of MBSP from various aspects, confirmed the anti-inflammatory effect of MBSP in vitro , and further verified the in vivo anti-inflammatory activity of MBSP using UC zebrafish as an animal model. The results of this study provide a reference for the development and application of MBSP in the field of treating inflammatory diseases. [ABSTRACT FROM AUTHOR]