Seasonal movements between main stem and tributaries may facilitate the persistence of Roundtail Chub and Flannelmouth Sucker within an altered stream system.

Objective: Movement enables animals to complete their life history by responding to changing environmental conditions. Linking movement behaviors to life history characteristics can allow more targeted management applications for declining native fish populations. We identified seasonal movement patterns of Roundtail Chub Gila robusta and Flannelmouth Sucker Catostomus latipinnis, two understudied species that currently occupy only a portion of their historical range in the Colorado River basin. Methods: We coupled passive integrated transponder tag antenna systems with multistate capture–recapture models to quantify juvenile and adult movement between main‐stem and tributary habitats in the Blacks Fork subbasin of southwestern Wyoming during 2019–2021. We also evaluated how flow and temperature may cue the timing of seasonal movements. Result: Adults from both species made spring movements, likely associated with spawning, to reach upstream tributary habitat, though adult Flannelmouth Sucker movements were more common and longer. Roundtail Chub primarily moved into the Hams Fork, while Flannelmouth Sucker primarily moved into Muddy Creek, an intermittent tributary that was also identified as important for juvenile rearing. Juvenile movements occurred primarily during the fall months, with distance traveled comparable between species. Temperature and flow influenced the timing of spring movements for adult Flannelmouth Sucker, with rising temperatures triggering movement and low flow potentially limiting access to preferred spawning habitat. Conclusion: Identified movements likely contribute to Roundtail Chub and Flannelmouth Sucker persistence in this highly altered stream system and ultimately provide insights for management and recovery strategies to prevent further population declines. Impact statementNative fish make seasonal movements into tributary habitats that likely allow them to track habitat suitable for spawning and juvenile rearing. [ABSTRACT FROM AUTHOR]