Characterization of hemocyte types in Bombyx mori throughout pupa, larva, and adult stages by light microscopy.

Hemocytes are specialized hemolymph cells found in insects, responsible for a variety of important physiological functions such as immunity, wound healing, and the regulation of the insect's circulatory system. The study of insect hemocytes has become increasingly important in recent years due to the emergence of insect-borne diseases and the need to develop new strategies for insect control. Understanding the biology of insect hemocytes has the potential to lead to the development of novel insecticides and strategies for controlling insect-borne diseases. This study aimed to examine the hemocytes in Bombyx mori to determine if there were any variations in the types of hemocytes to other arthropods. By comprehensively characterizing hemocyte types across developmental stages, this research sheds light on the intricate immune responses and adaptations of the silkworm. Such insights could contribute to the understanding of basic immunological mechanisms in insects. Hemolymph of B. mori was collected, and hemolymph smears were stained with Wright’s stain. In B. mori, four distinct types of hemocyte prohemocyte, plasmatocyte, granulocyte, and oenocytoid were examined. Using light microscopy, the hemocyte types were identified based on their size, the presence or absence of granules, and the ratio of their nucleus to cytoplasm. [ABSTRACT FROM AUTHOR]