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Ouabain, ATPase inhibitor, potentially enhances the effect of polyhexamethylene biguanide on Acanthamoeba castellanii.

Authors :
Shih, Kuang-Yi
Chang, Yao-Tsung
Wang, Yu-Jen
Huang, Jian-Ming
Source :
International Journal for Parasitology: Drugs & Drug Resistance; Aug2024, Vol. 25, pN.PAG-N.PAG, 1p
Publication Year :
2024

Abstract

Acanthamoeba , a free-living amoeba, is commonly found in various natural environments, such as rivers and soil, as well as in public baths, swimming pools, and sewers. Acanthamoeba can cause severe illness such as granulomatous amoebic encephalitis and Acanthamoeba keratitis (AK) in humans. AK, the most recognized disease, can cause permanent visual impairment or blindness by affecting the cornea. AK commonly affects contact lens wearers who neglect proper cleaning habits. The symptoms of AK include epithelial and stromal destruction, corneal infiltrate, and intense ocular pain, occasionally necessitating surgical removal of the entire eyeball. Current AK treatment involves the hourly application of eye drops containing polyhexamethylene biocide (PHMB). However, studies have revealed their ineffectiveness against drug-resistant strains. Acanthamoeba can form cysts as a survival mechanism in adverse environments, though the exact mechanism remains unknown. Our experiments revealed that sodium P-type ATPase (ACA1_065450) is closely linked to encystation. In addition, various encystation buffers, such as MgCl 2 or NaCl, induced the expression of P-type ATPase. Furthermore, we used ouabain, an ATPase inhibitor, to inhibit the Na<superscript>+</superscript>/K<superscript>+</superscript> ion pump, consequently decreasing the encystation rate of Acanthamoeba. Our primary objective is to develop an advanced treatment for AK. We anticipate that the combination of ouabain and PHMB may serve as an effective therapeutic approach against AK in the future. [Display omitted] • The expression of the sodium P-type ATPase gene increase after encystation. • Ouabain inhibits encystation on A. castellanii. • Effect of a Combination drug treatment on A. castellanii. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
22113207
Volume :
25
Database :
Supplemental Index
Journal :
International Journal for Parasitology: Drugs & Drug Resistance
Publication Type :
Academic Journal
Accession number :
178856906
Full Text :
https://doi.org/10.1016/j.ijpddr.2024.100550