Research Data from Northeastern University Update Understanding of Eubacterium (An Efficient Cre-based Workflow for Genomic Integration and Expression of Large Biosynthetic Pathways In eubacterium Limosum).

Researchers from Northeastern University have developed a new method for integrating and expressing large gene clusters in the human gut acetogen Eubacterium limosum. This method, called chassis-independent recombinase-assisted genome engineering (CRAGE), allows for the production of phevalin, a non-ribosomal peptide synthetase, in E. limosum. The study highlights the potential of E. limosum as a chassis for exploring anaerobic natural product biosynthesis and expands the molecular toolkit for Clostridia, a group of bacteria with biosynthetic potential. The research was funded by Northeastern University and the Advanced Research Projects Agency - EnergyA. [Extracted from the article]