Development of inducible promoter and CRISPRi plasmids functional in Rickettsia rickettsii.

A preprint abstract from biorxiv.org discusses the development of genetic tools to study Rickettsia rickettsii, a tick-borne bacterium that causes Rocky Mountain spotted fever. The researchers describe the construction of an inducible promoter system and a CRISPR interference plasmid to regulate gene expression in R. rickettsii. They demonstrate the functionality of these tools and their potential applications in disrupting the expression of specific genes. This research has not yet undergone peer review. [Extracted from the article]