Generation and identification of a resistance to stripe rust perennial intergeneric hybrid F1 between Roegneria ciliaris and common wheat.

In this study, we evaluated the stripe rust resistance type and estimated the published resistance genes in 13 Roegneria species including 29 materials. Afterward, we conducted artificial crosses and investigated the morphology characters, genome constitution, and stripe rust resistance of the intergeneric hybrid F₁ between stripe rust resistance Roegneria material and common wheat. The results showed as follows: 82.76% of the 29 tested materials exhibited stripe rust resistance at adult stage, and they contained more than 5 alleles of stripe rust resistance genes, which might still carry new resistance genes to wheat stripe rust. An intergeneric hybrids R. ciliaris-CSph2a F₁ was generated using the screened high stripe rust resistance Roegneria ciliaris [Trin.] Nevski (ZY11004-R) and common wheat mutant CSph2a, and raised based on the embryo rescue technology. F₁ hybrid contained 35 chromosomes with StYABD genome constitution and an average of 26.84 monovalents during pollen mother cell metaphase I. Besides, F₁ exhibited morphological intermediacy, except the perennial living was inherited form from maternal R. ciliaris with high resistance to wheat stripe rust. [ABSTRACT FROM AUTHOR]