A multiplex method for the detection of food microorganisms based on suspension microarrays combined with recombinase-aided amplification.

Foodborne diseases caused by foodborne pathogenic microorganisms have long been a severe public health problem. Timely and accurate detection of food-borne microorganisms can minimize their harm and enable the development of efficient control strategies. In this study, we establish a multiplex nucleic acid detection method based on recombinase-aided amplification reaction on a suspension microarray (referred to as SC-RAA) to identify and quantify microorganisms. The experimental results confirmed that this approach has an outstanding sensitivity, with a limit of detection (LOD) of 0.8 fM and a linear range of 0.8 fM–80 pM, spanning five orders of magnitude. Moreover, the prepared method also showed exquisite specificity, which the amplified signal can be detected only when the target nucleic acid is present. The recovery rate of the SC-RAA assay was 82.2%–113.4%, suggesting that the SC-RAA assay is performing well and accurately measuring the concentration of the substance in the sample. In addition, the consistency analysis showed that the results of the SC-RAA detection were also in good agreement with those of traditional qPCR detection (R² > 0.95). These results highlight the potential of the SC-RAA assay to be applied as a multiplex and sensitive DNA detection platform for identifying and quantifying foodborne pathogenic microorganisms. [ABSTRACT FROM AUTHOR]