Therapeutic effect of human umbilical cord mesenchymal stem cells and their conditioned medium on LPS-induced endometritis in mice.

To explore the effect of human umbilical cord mesenchymal stem cells (hUC-MSCs) and their conditioned medium (MSC-CM) in repairing the endometritis mouse model in vivo. Lipopolysaccharide (LPS) was used to induce acute inflammation in endometritis mouse model. Mice were treated in six groups: control group (PBS), model group (LPS), LPS+MSC-CM (6 h) group, LPS+MSC-CM (12 h) group, LPS+MSCs (6 h) group and LPS+MSCs (12 h) group. Morphological and histological changes of mouse uterus were observed, and mouse uterine inflammation index myeloperoxidase (MPO) and related immune index TNF-α, IL-6 and IL-1β levels were detected by ELISA. There exist remarkable inflammatory response and an obvious increase in the value of MPO, TNF-α, IL-1β and IL-6 in the endometritis mouse model compared with the control group. Morphological and histological appearances were relieved after treated with hUC-MSCs and MSC-CM. Besides, the value of MPO, TNF-α, IL-1β and IL-6 showed different degrees of decline. In comparison with LPS+MSC-CM (12 h) and LPS+MSCs (12 h) group, there was significant decrease in inflammatory indicators in LPS+MSC-CM (6 h) and LPS+MSCs (6 h) group. Intrauterine infusion of hUC-MSCs and MSC-CM can alleviate LPS induced endometritis. • hUC-MSCs possess distinct advantages in the treatment of endometritis. • Conditioned medium of hUC-MSCs is abundant in nutrients, chemokines, cytokines, and enzymes secreted, which can be used for disease treatment. • hUC-MSCs and its conditioned medium are effect in treating endometritis. [ABSTRACT FROM AUTHOR]