DNA PLOIDY COMPARATIVE ANALYSIS BETWEEN THE DIFFERENT STAGES OF PLEOMORPHIC ADENOMA AND ITS MALIGNANT COUNTERPART.

To compare the DNA ploidy profile between the pleomorphic adenoma (PA) in the different clinical stages of its malignant transformation. Forty-six samples divided into the normal salivary gland (control), primary PA, recurrent PA, residual PA, and CXPA were used. Flow cytometric analysis was performed to evaluate the cell percentage among the cell cycle phases. All cell cycle phases showed a statistical difference between the study groups (G1: p<0.001; G2: p=0.009; S: p=0,009; 5cER: p=0,002). In the G1 phase, CXAP obtained a decrease in the percentage of cells compared to primary AP (p=0.003) and recurrent AP (p<0.001) and residual AP showed a decrease compared to recurrent AP (p=0.004). In the S phase, there was a statistical difference between the amount of CXAP and recurrent AP nuclei (p=0.01). Recurrent AP also demonstrated a lower percentage compared to residual (p=0.015) and CXAP (p=0.007) in the 5cER phase. The significant change in the DNA ploidy profile was shown to be a potential differential feature during the malignant progression of PA, while among the frankly benign lesions, no significant changes were observed. [ABSTRACT FROM AUTHOR]