Kasetsart University Researchers Detail New Studies and Findings in the Area of African Swine Fever Virus (Small ubiquitin-like modifier-tag and modified protein purification significantly increase the quality and quantity of recombinant...).

Researchers from Kasetsart University in Bangkok, Thailand have conducted a study to improve the solubility, yield, and purity of recombinant p30, a protein associated with African swine fever virus (ASFV). They achieved this by tagging the p30 protein with a small ubiquitin-like modifier (SUMO) and modifying the protein purification process. The study found that the solubility and expression levels of the SUMO-tagged p30 were significantly enhanced compared to the untagged p30. The modified purification process also increased the yield of purified p30. These findings have implications for the production of other recombinant proteins and could be used in immunization and diagnostic purposes. [Extracted from the article]