Highly sensitive mapping of in vitro type II topoisomerase DNA cleavage sites with SHAN-seq.

A recent preprint abstract discusses the development of a new method called SHAN-seq, which allows for the sensitive mapping of type II topoisomerase DNA cleavage sites in vitro. Type II topoisomerases are enzymes that create breaks in DNA, and the location and frequency of these breaks are important for cellular function and the effectiveness of certain drugs. Using SHAN-seq, researchers were able to detect hundreds of cleavage sites on DNA, including both previously known sites and new sites. The study also found that the location and frequency of these cleavage sites varied depending on the specific enzyme and the presence of certain drugs. This research provides a more detailed understanding of how these enzymes function and could have implications for the development of new drugs. However, it is important to note that this preprint has not yet undergone peer review. [Extracted from the article]