In Vitro ANTIBACTERAIL ACTIVITY AND REDUCED BIOFILM FORMATION OF HONEY AGAINST Pseudomonas Aeruginosa AND Staphylococcus Aureus.

The need to investigate new pharmaceutical compounds, particularly natural molecules, as potential alternatives to synthetic chemicals compounds in order to address the issues of biofilms development and antibiotic resistance in pathogenic bacteria. Honey, is known to have both nutritional and therapeutic virtues and a real reservoir of functional molecules. This research aimed to evaluate the antibacterial and antibiofilm formation properties of two honey samples (Multifloral honey and Sidr honey) against Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 25923. The evaluation of the antimicrobial activity of these two honey samples was made using well assay and the Minimum Inhibitory Concentration (MIC) was determined using the micro-broth dilution assay showed that Multifloral honey and Sidr honey exhibit an antibacterial effect against both tested bacteria. The inhibition zones revealed that the tested bacteria did not express the same sensitivity where a high activity was recorded with multifloral honey against S. aureus. These results were confirmed by the MIC values, where Staphylococcus aureus was more sensitive and it was inhibited at 6.25% and 12.5% with Multifloral and Sidr honey respectively, while Pseudomonas aeruginosa was inhibited at a concentration of 25% (v/v) with both honey types. According to this study, we revealed also that the biofilms formation of Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 25923 were decreased when incubated with the sub-inhibitory concentration of both tested honey samples. [ABSTRACT FROM AUTHOR]