Nagasaki University Researchers Report on Findings in Gene Therapy (Interaction of g-Polyglutamic Acid/Polyethyleneimine/Plasmid DNA Ternary Complexes with Serum Components Plays a Crucial Role in Transfection in Mice).

A recent study conducted by researchers at Nagasaki University in Japan explores the use of non-viral vectors in gene therapy. The study focuses on the interaction between serum components and gene transfer using polyethyleneimine (PEI) and g-polyglutamic acid (g-PGA) complexes. The researchers found that heat-labile components in the serum contribute to gene expression in binary complexes, while serum albumin and salts affect gene expression in ternary complexes. The study suggests that changes in particle size and z-potential may play a role in enhancing gene expression. These findings provide valuable insights into the use of polymers like PEI and g-PGA in non-viral gene transfer. [Extracted from the article]