"Bacterial Dna Cytosine Deaminases For Mapping Dna Methylation Sites" in Patent Application Approval Process (USPTO 20240124867).

The University of Washington has filed a patent application for a method of detecting methylation of cytosine residues in DNA. The method involves using bacterial cytosine deaminases to deaminate unmethylated cytosine residues in a polynucleic acid molecule. The inventors provide details on the specific bacterial cytosine deaminases that can be used, as well as the concentration at which they should be present in the reaction. The method also includes steps for isolating the DNA from a biological sample and detecting the occurrence of deamination events. The application also describes a kit that includes a bacterial cytosine deaminase and reagents for facilitating the deamination process. [Extracted from the article]