Studies in the Area of Chemicals and Chemistry Reported from Centre for Cooperative Research in Biomaterials (A Study of Complexation and Biological Fate of Polyethyleneimine-sirna Polyplexes In Vitro and In Vivo By Fluorescence Correlation...).

Researchers at the Centre for Cooperative Research in Biomaterials in Donostia San Sebastian, Spain, have conducted a study on the formation and biological fate of polymer-based gene vectors. They formed polyplexes of polyethyleneimine (PEI) and silencing RNA (siRNA) with different theoretical N/P ratios. The researchers used fluorescence correlation spectroscopy (FCS) to study the formation of polyplexes and determine their stoichiometry. They also used positron emission tomography (PET) to study the biodistribution of the polyplexes in healthy mice. The study found that the polyplexes disassemble after 1 day inside cells and show limited circulation in the bloodstream and accumulation in the liver. Higher activity of PEI in the kidney and bladder suggests the presence of free PEI. This research provides valuable insights into the development of gene therapy. [Extracted from the article]