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Protective effect of melatonin on blood-brain barrier damage caused by Endotoxemia.

Authors :
Kalkan, Kubra Tugce
Esrefoglu, Mukaddes
Terzioglu-Usak, Sule
Yay, Arzu
Source :
Neurological Research; Feb2024, Vol. 46 Issue 2, p195-206, 12p
Publication Year :
2024

Abstract

Endotoxins, products of Gram-negative bacteria, are the primary cause of blood-brain barrier (BBB) damage. In the present study, we aimed to investigate the possible neuroprotection mechanisms of melatonin on BBB damage induced by endotoxemia. Adult, female Sprague-Dawley rats (n = 42) were separated into four random groups as a control group and three treatment groups. Lipopolysaccharide (7,5 mg/kg/day) was administrated for a single dose to generate a 24-hour sepsis model on rats. Melatonin (10 mg/kg/day) was treated a week before sepsis. Afterward, the dissected brain tissues were examined by histopathological, biochemical, and molecular analyses. LPS caused weight loss in the groups. As a result, degenerated neurons with cytoplasmic vacuoles and irregular pyknotic nuclei, pale stained necrotic neurons, and vascular congestion were observed in LPS-exposed rats. However, MEL decreased the number of degenerated neurons in treated groups. MEL treatment increased ZO1 and Occludin immunoreactivity while decreasing TLR4 in brain tissues. MEL effect on protein expression was recorded for ZO1 increase and TLR4 decrease in brain tissue compared to LPS groups. MEL also decreased MDA levels in brain tissue. MEL recovered the degenerative damage of sepsis by contributing to blood-brain barrier integrity, and by decreasing inflammation, thus the neuroprotective effects of MEL might provide an experimental basis for clinical applications. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
01616412
Volume :
46
Issue :
2
Database :
Supplemental Index
Journal :
Neurological Research
Publication Type :
Academic Journal
Accession number :
174583034
Full Text :
https://doi.org/10.1080/01616412.2023.2265244