Modification of microfiltration membranes by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride/n-hydroxy sulfosuccinimide coupling mediated antimicrobial peptides grafting for biofouling mitigation.

Microfiltration membrane separation is widely applied for water and wastewater but biofouling is a challenging task in real applications. In this work, we fabricated a novel antibiofouling polyvinylidene fluoride (PVDF) membrane by grafting non-drug-resistant antimicrobial peptides (AMPs) on the membranes via 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride/ N -hydroxy sulfosuccinimide (EDC/Sulfo-NHS) chemistry. Effects of AMPs concentration on the membrane basic properties, antibacterial and antibiofouling behaviors of the modified membranes were systematically investigated. Membrane surface characterization demonstrated that the AMPs were successfully grafted onto the base PVDF membranes. The AMPs modified membrane showed enhanced hydrophilicity, consistent permeability and prominent antimicrobial ability (i.e., >90% of antibacterial efficiency against both Escherichia coli and Staphylococcus aureus). Batch filtration tests exhibited that the modified membranes with the concentration of AMPs less than or equal to 0.7 mg/mL had lower flux decline rate compared to the pristine membrane (M0). According to the images from confocal laser scanning microscopy analysis, less total cells as well as more dead cells were observed on the AMPs modified membrane compared to M0. Furthermore, the grafted AMPs on the membranes also displayed favorable stability suffered from a cycle involving biofouling and chemical cleaning. This grafting protocol for AMPs highlights a robust way to fabricate antibiofouling membranes for water and wastewater treatment. [Display omitted] • Grafting antimicrobial peptides (AMPs) on a membrane for biofouling mitigation. • AMPs grafted membranes showed antibacterial effects against S. aureus and E. coli. • AMPs grafted membranes demonstrated compelling antibiofouling performance. • AMPs grafted membranes exhibited favorable stability after chemical cleaning. • It provides a new way to fabricate antibiofouling membranes for water treatment. [ABSTRACT FROM AUTHOR]