Development of a functional dairy snack containing oleoylethanolamide that reduces food intake in normal-weight and obese minipigs.

[Display omitted] • Oleoylethanolamide (OEA) exhibit promising anorexigenic properties. • Food structure may modulate the kinetic of OEA release. • Liquid, but not solid, OEA-containing dairy snack reduced feed intake in minipigs. • OEA release differed between snack structures during in vitro digestion tests. • Different anorexigenic mechanisms seem at play depending on minipig weight status. Oleoylethanolamide (OEA) is a safe bioactive lipid that demonstrated strong anorexigenic properties in preclinical and clinical models. In order to evaluate the importance of OEA delivery kinetic on its anorectic properties, we developed OEA-containing dairy snacks with either a liquid or a semi-solid form. The OEA+ liquid snack, but not the semi-solid one, reduced by 14 and 18 % the amount of feed eaten by normal-weight and obese minipigs, respectively, in an eating behavior test performed 4 h after snack ingestion. In vitro digestion experiments revealed that OEA release in intestinal digesta was greatly enhanced when the snack was liquid compared to the semi-solid structure. Kinetic investigations of several plasma parameters after liquid snack ingestion points towards different potential mechanism depending upon the minipig weight status, with an effect of the OEA+ liquid snack likely on endocannabinoid and other related N-acylethanolamine metabolism in normal-weight minipigs and on ketogenesis in obese ones. [ABSTRACT FROM AUTHOR]