Black soldier fly larvae recruit functional microbiota into the intestines and residues to promote lignocellulosic degradation in domestic biodegradable waste.

Lignocellulose is an important component of domestic biodegradable waste (DBW), and its complex structure makes it an obstacle in the biological treatment of DBW. Here, we identify black soldier fly larvae (Hermetia illucens L., BSFL) as a bioreactor for lignocellulose degradation in DBW based on their ability to effectively recruit lignocellulose-degrading bacteria. This study mainly examined the lignocellulose degradation, dynamic succession of the microbial community, gene expression of carbohydrate-active enzymes (CAZymes), and co-occurrence network analysis. Investigation of lignocellulose degradation by BSFL within 14 days indicated that the lignocellulose biodegradation rate in the larvae treatment (LT, 26.5%) group was higher than in natural composting (NC, 4.06%). In order to gain a more comprehensive understanding of microbiota, we conducted metagenomic sequencing of larvae intestines (LI), along with the LT and NC. The relative abundance of lignocellulose-degrading bacteria and CAZymes genes in LT and LI were higher than those in NC based on metagenomics sequencing. Importantly, genes coding cellulase and hemicellulase in LI were 3.36- and 2.79-fold higher, respectively, than that in LT, while the ligninase genes in LT were 1.82-fold higher than in LI. A co-occurrence network analysis identified Enterocluster and Luteimonas as keystone taxa in larvae intestines and residues, respectively, with a synergistic relationship to lignocellulose-degrading bacteria. The mechanism of recruiting functional bacteria through the larvae intestines promoted lignocellulose degradation in DBW, improving the efficiency of BSFL biotechnology and resource regeneration. [Display omitted] • BSFL recruited lignocellulose degrading functional bacteria in gut-residue system. • BSFL enriched with functional bacteria improved lignocellulose degradation by 526%. • Corynebacterium and Brevibacterium recruited mainly in residues to degrade lignin. • Dysgonomonas, Prevotella, and Miniimonas recruited in gut to degrade celluloses. • Enterocluster and Luteimonas as keystone taxa in BSFL intestines and residues. [ABSTRACT FROM AUTHOR]