Back to Search
Start Over
Production of functional Arabica and Robusta green coffee beans: Optimization of fermentation with microbial cocktails to improve antioxidant activity and metabolomic profiles.
- Source :
- Biocatalysis & Agricultural Biotechnology; Oct2023, Vol. 53, pN.PAG-N.PAG, 1p
- Publication Year :
- 2023
-
Abstract
- Currently, growing recognition of the health benefits associated with functional foods and beverages has increased the demand for products that offer more than just basic nutrition. Coffee is a widely consumed beverage, in which fermentation of green coffee beans contributes to the development of a special aroma and higher levels of antioxidants. The objectives of this study were to optimize the fermentation conditions and simultaneously improve antioxidant activity and metabolites. The conditions optimized by Box–Behnken design (BBD) suggested that the highest total esters, total phenolic content (TPC), and antioxidant activity (ABTS, DPPH, FRAP) could be reached by fermentation at 30 °C for 2 days with 13.25% sucrose (w/w) using a microbial cocktail. Two sets of selective microbial starters, Lactiplantibacillus plantarum TISTR 2265 (LP) and a cocktail of TISTR yeasts with LP (YC-LP), were further considered as powerful starters for solid-state fermentation (SSF) of Arabica and Robusta beans, enhancing antioxidant activity within the first 3 days of fermentation. Arabica beans fermented by LP reached 198.03% TPC, 44.21% ABTS, 90.35% DPPH, and 97.13% FRAP, while Robusta beans fermented by YC-LP showed 108.24% TPC, 204.19% ABTS, 170.71% DPPH, and 59.60% FRAP, higher than an uninoculated starter. The analysis of 41 fermented metabolites revealed the different metabolomic profiles for both coffee beans, offering valuable insight into the correlation between chemical constituents and functional characteristics. This study represents the first comprehensive comparison of the metabolomic profiles of fermented Arabica and Robusta green coffee beans. [Display omitted] • Functional green coffee beans were produced using microbial cocktail starters. • The optimal condition for the functional beans was incubation for 2 days at 30 °C and 13.25% sucrose. • High functional value of green Robusta coffee beans was produced using YC and LP (YC-LP). • Comparative metabolomic profiles of fermented green Arabica and Robusta beans were investigated and the 41 metabolites were observed by using UHPLC-Orbitrap-MS. • Types of microbial starters influenced the chemical and functional characteristics in the process. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 18788181
- Volume :
- 53
- Database :
- Supplemental Index
- Journal :
- Biocatalysis & Agricultural Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 173456102
- Full Text :
- https://doi.org/10.1016/j.bcab.2023.102869