Back to Search
Start Over
Benzo[a]pyrene-induced up-regulation of circ_0003552 via ALKBH5-mediated m6A modification promotes DNA damage in human bronchial epithelial cells.
- Source :
- Environmental Pollution; Nov2023, Vol. 336, pN.PAG-N.PAG, 1p
- Publication Year :
- 2023
-
Abstract
- Benzo [a]pyrene (B [a]P) is a widespread environmental chemical pollutant that has been linked to the development of various diseases. However, the specific mechanism of action remains unclear. In this study, human bronchial epithelial 16HBE and BEAS-2B cells were exposed to B [a]P at 0–32 μM to assess the DNA-damaging effects. B [a]P exposure resulted in elevated expression of γ-H2AX, a marker of DNA damage. The m<superscript>6</superscript>A RNA methylation assay showed that B [a]P exposure increased the extent of m<superscript>6</superscript>A modification and the demethylase ALKBH5 played an integral role in this process. Moreover, the results of the comet assay and Western blot analysis showed an increase in m<superscript>6</superscript>A modification mediated by ALKBH5 that promoted DNA damage. Furthermore, the participation of a novel circular RNA, circ_0003552, was assessed by high-throughput sequencing under the condition of high m<superscript>6</superscript>A modification induced by B [a]P exposure. In subsequent functional studies, an interference/overexpression system was created to confirm that circ_0003552 participated in regulation of DNA damage. Mechanistically, circ_0003552 had an m<superscript>6</superscript>A binding site that could regulate its generation. This study is the first to report that B [a]P upregulated circ_0003552 through m<superscript>6</superscript>A modification, thereby promoting DNA damage. These findings revealed that epigenetics played a key role in environmental carcinogen-induced DNA damage, and the quantitative changes it brought might provide an early biomarker for future medical studies of genetic-related diseases and a new platform for investigations of the interaction between epigenetics and genetics. [Display omitted] • B [a]P induces DNA damage in bronchial epithelial cells. • B [a]P upregulates the degree of m<superscript>6</superscript>A modification by suppressing ALKBH5 expression. • Both ALKBH5 and circ_0003552 can regulate the B [a]P-induced DNA damage. • M<superscript>6</superscript>A modification promotes circ_0003552 production through the m<superscript>6</superscript>A binding site. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 02697491
- Volume :
- 336
- Database :
- Supplemental Index
- Journal :
- Environmental Pollution
- Publication Type :
- Academic Journal
- Accession number :
- 172776816
- Full Text :
- https://doi.org/10.1016/j.envpol.2023.122367