Patent Application Titled "Production Bacterial Cells And Use Thereof In Production Methods" Published Online (USPTO 20230279364).

"This approach represents a novel avenue for easier and/or safer generation of phage particles and/or phage-derived delivery vehicles, targeting bacterial cells known to be pathogenic and/or difficult to manipulate and/or inefficient to use in phage particles and/or phage-derived delivery vehicles production for any reason. "The present invention also concerns a method for producing phage particles or phage-derived delivery vehicles, comprising: " "(a) providing the production bacterial cell of the invention, and "(b) inducing, in said production bacterial cell, expression of said at least one of said phage structural gene(s) and phage DNA packaging gene(s), and assembly of the products expressed by said at least one phage structural gene(s) and said at least one phage DNA packaging gene(s), thereby producing phage particles or phage-derived delivery vehicles. " "Another object of the invention concerns a hybrid helper phage system comprising: " "(i) at least one phage DNA packaging gene(s) derived from a first type of bacteriophage, "(i') at least one phage structural gene(s) derived from said first type of bacteriophage, and "(ii) at least one gene, derived from a second type of bacteriophage, involved in phage excision/insertion, phage DNA replication, and/or phage regulation, " "wherein said genes (i), (i') and (ii) are comprised in a unique nucleic acid molecule or in separate nucleic acid molecules, "wherein said first type of bacteriophage comes from and/or target bacterial species or strain different from the bacterial species or strain from which said second type of bacteriophage comes and/or that said second type of bacteriophage targets, and "wherein said hybrid helper phage system does not comprise any expressed phage structural gene derived from said second type of bacteriophage.". [Extracted from the article]