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In-Vitro, In-Vivo AND In-Silico ANTIMICROBIAL ASSESSMENT OF Bacillus Subtilis (RB14) ITURIN A AGAINST Fusarium Graminearum MYOSIN I PROTEIN.

Authors :
Khan, Muddasir
Salman, Muhammad
Ali, Sher
Abdullah
Slama, Petr
Skladanka, Jiri
Horky, Pavel
Source :
Fresenius Environmental Bulletin; Jun2023, Vol. 32 Issue 6, p2428-2435, 8p
Publication Year :
2023

Abstract

In this study Bacillus subtilis RB14 iturin A was screened by in-vitro, in-vivo, and in-silico approaches against Fusarium graminearum, causing a major threat to worldwide crop production. Lipopeptide iturin A was characterized by highperformance liquid chromatography (HPLC). Iturin A showed successful inhibitory potential (p<0.005) against a growing F. graminearum in varied concentrations (1.00, 0.50, 0.25, 0.125 M). Nevertheless, the lipopeptide ext ract revealed an upsurged growth in plant's height, weight, and roots weight. Evidently, such spp. prominently reduces the incidence of F. graminearum (23%). Moreover, in computational analysis, the iturin A has a -6.7 kcal/mol b inding affinity for myosin I protein, and due to this it inhibited the production of F. graminearum deoxynivalenol (DON) mycotoxin. To this an end, B. subtilis RB14 can be classified as a good biological candidate and capably produces a variety of biomarkers to diminish deleterious phytopathogens and can be used as biopesticide. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
10184619
Volume :
32
Issue :
6
Database :
Supplemental Index
Journal :
Fresenius Environmental Bulletin
Publication Type :
Academic Journal
Accession number :
164453532