Curcumin alleviates lipid deposition in hepatocytes through miR-3666/AMPK axis regulation.

Curcumin (CUR), the main compound extracted from Curcuma longa , has various biological actions, including hepatoprotective, anti-infective, anti-inflammatory, antioxidant, and neurotrophic properties. But it has not yet been clarified how CUR affects the accumulation of fatty in hepatic cells. Our study uncovered that CUR decreased the lipid deposition in free fat acids (FFAs)-treated hepatocytes, which could be blocked by Compound C (an AMPK inhibitor). This result suggests that protective effect was associated with the AMPK pathway activation. MiR-3666 directly targets the AMPK gene, and overexpression of miR-3666 greatly weakens the hypolipidemic effect of CUR. Our results showed that CUR reduced FFAs-induced lipid deposition by targeting miR-3666. This boosted AMPK phosphorylation and inhibited its downstream targets, including ACC and SREBP1. These findings together confirm that CUR-mediated inhibition of miR-3666 to activate the AMPK pathway is a potential alternative for alleviation of hepatic lipid deposition. [Display omitted] • CUR has the capacity to alleviate lipid deposition induced by FFAs in HepG2 cells. • CUR improved AMPK levels and its downstream targets in FFAs-treated HepG2 cells. • CUR inhibited miR-3666 expression to activate the AMPK pathway. • CUR treatment prevented lipid deposition through regulation of miR-3666/AMPK. [ABSTRACT FROM AUTHOR]