Transcriptome analysis of anorexic and preferred silkworms (Bombyx mori) on artificial diet.

The silkworm, Bombyx mori , is an important oligophagous economic insect and feeding habits of different silkworm varieties to artificial diet are different. Research on the mechanisms of feeding habits on artificial diet, and breeding of silkworm varieties adapted on artificial diet, which is a necessary condition for industrial silkworm rearing, is currently lacking. For an artificial diet, Xin was anorexic, whereas Haoyue A showed a strong appetite. When the two varieties were crossed, the F 1 generation showed a poor appetite for the artificial diet and had a setae dispersion rate of <50 %. However, the F 2 generation, self-bred progeny of F 1 , had a good appetite for the artificial diet, with a setae dispersion rate of 70 %. Herein, transcriptome analysis was conducted on the F 2 generation, comparing individuals with anorexic and preferred feeding habits, and 2188 differential genes were identified, with 1524 genes up-regulated and 934 genes down-regulated. Several genes were identified to contribute to feeding habits, such as genes involved olfactory system, energy supply, and cell proliferation and differentiation. GO enrichment revealed a large number of DEGs related to behavior, growth, signaling, developmental process, response to stimulation, and other pathways. Furthermore, proteins closely related to feeding were expressed differently. Some DEGs were selected for qRT-PCR, and results indicated the reliability of the DEG results. The DEGs between individuals with anorexic and preferred feeding habits were screened by RNA-Seq technology, which provides a reliable reference to study molecule mechanisms of feeding habits on artificial diet. We compared the different expression genes between anorexic individuals and preferred individuals to artificial diet. We annotated and classified these unigene sequences by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional classification. Significant differences and pathways may be related to feeding habit of silkworm to artificial diet were found in anorexic individuals. Our study provides basic data for the breeding of silkworm varieties adapted to artificial diet and insights into feeding habit mechanism. [Display omitted] [ABSTRACT FROM AUTHOR]