Weizmannia acidilactici sp. nov., a lactic acid producing bacterium isolated from soils.

[Display omitted] • Three novel spore-forming bacteria, designated strains PP-18^T, JC-4, and JC-7, isolated from soils in Thailand, were taxonomically characterized using polyphasic approach. • Weizmannia acidilactici sp. nov. is proposed for PP-18^T, JC-4 and JC-7 based on 16S rRNA gene sequence similarity, ANI, and DNA-DNA hybridization (dDDH) values compared to those of W. coagulans LMG 6326^T and W. acidiproducens KCTC 13078^T. • Weizmannia strains PP-18^T, JC-4 and JC-7 produced l -lactic acid from glucose in the range of 45.35–47.75 g/L with the yield of 0.63–0.76 g/g and productivity of 0.63–0.66 g/L·h. The strains designed PP-18^T, JC-4 and JC-7 isolated from soils, were Gram-stain-positive rods, facultative anaerobe, endospore-forming bacteria. The strains produced l -lactic acid from glucose. They showed positive for catalase but negative for oxidase, nitrate reduction and arginine hydrolysis. Strains P-18^T, JC-4 and JC-7 were closely related to Weizmannia coagulans LMG 6326^T (97.27–97.64%) and W. acidiproducens KCTC 13078^T (96.46–96.74%) based on 16S rRNA gene sequence similarity, respectively. They contained meso -diaminopimelic acid in cell wall peptidoglycan and had seven isoprene units (MK-7) as the predominant menaquinone. The major cellular fatty acids of strain PP-18^T were iso -C 15:0 , anteiso-C 17:0 , iso-C 16:0 and anteiso-C 15:0. The ANIb and ANIm values among the genomes of strains PP-18^T, JC-4 and JC-7 are above 99.4% while their ANIb and ANIm values among them and W. coagulans LMG 6326^T and W. acidiproducens KCTC 13078^T were ranged from 76.61 to 79.59%. These 3 strains showed the digital DNA-DNA hybridization (dDDH) values of 20.7–23.6% when compared with W. coagulans LMG 6326^T and W. acidiproducens DSM 23148^T. The DNA G + C contents of strains PP-18^T, JC-4 and JC-7 were 45.82%, 45.86% and 45.86%, respectively. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphoglycolipids. The results of phenotypic and chemotaxonomic characteristics and whole-genome analysis indicated that the strains PP-18^T, JC-4 and JC-7 should be represented as a novel species within the genus Weizmannia for which the name Weizmannia acidilactici sp. nov. is proposed. The type strain is PP-18^T (=KCTC 33974^T = NBRC 113028^T = TISTR 2515^T). [ABSTRACT FROM AUTHOR]