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H-NS Represses Biofilm Formation and c-di-GMP Synthesis in Vibrio parahaemolyticus.

Authors :
XUE, Xing Fan
ZHNAG, Miao Miao
SUN, Jun Fang
LI, Xue
WU, Qi Min
YIN, Zhe
YANG, Wen Hui
NI, Bin
HU, Ling Fei
ZHOU, Dong Sheng
LU, Ren Fei
ZHANG, Yi Quan
Source :
Biomedical & Environmental Sciences; Sep2022, Vol. 35 Issue 9, p821-829, 9p
Publication Year :
2022

Abstract

This study aimed to investigate the regulation of histone-like nucleoid structuring protein (H-NS) on biofilm formation and cyclic diguanylate (c-di-GMP) synthesis in Vibrio parahaemolyticus RIMD2210633. Regulatory mechanisms were analyzed by the combined utilization of crystal violet staining, quantification of c-di-GMP, quantitative real-time polymerase chain reaction, LacZ fusion, and electrophoretic-mobility shift assay. The deletion of hns enhanced the biofilm formation and intracellular c-di-GMP levels in V. parahaemolyticus RIMD2210633. H-NS can bind the upstream promoter–proximal DNA regions of scrA, scrG, VP0117, VPA0198, VPA1176, VP0699, and VP2979 to repress their transcription. These genes encode a group of proteins with GGDEF and/or EAL domains associated with c-di-GMP metabolism. One of the mechanisms by which H-NS represses the biofilm formation by V. parahaemolyticus RIMD2210633 may be via repression of the production of intracellular c-di-GMP. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
08953988
Volume :
35
Issue :
9
Database :
Supplemental Index
Journal :
Biomedical & Environmental Sciences
Publication Type :
Academic Journal
Accession number :
159690803
Full Text :
https://doi.org/10.3967/bes2022.106