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H-NS Represses Biofilm Formation and c-di-GMP Synthesis in Vibrio parahaemolyticus.
- Source :
- Biomedical & Environmental Sciences; Sep2022, Vol. 35 Issue 9, p821-829, 9p
- Publication Year :
- 2022
-
Abstract
- This study aimed to investigate the regulation of histone-like nucleoid structuring protein (H-NS) on biofilm formation and cyclic diguanylate (c-di-GMP) synthesis in Vibrio parahaemolyticus RIMD2210633. Regulatory mechanisms were analyzed by the combined utilization of crystal violet staining, quantification of c-di-GMP, quantitative real-time polymerase chain reaction, LacZ fusion, and electrophoretic-mobility shift assay. The deletion of hns enhanced the biofilm formation and intracellular c-di-GMP levels in V. parahaemolyticus RIMD2210633. H-NS can bind the upstream promoter–proximal DNA regions of scrA, scrG, VP0117, VPA0198, VPA1176, VP0699, and VP2979 to repress their transcription. These genes encode a group of proteins with GGDEF and/or EAL domains associated with c-di-GMP metabolism. One of the mechanisms by which H-NS represses the biofilm formation by V. parahaemolyticus RIMD2210633 may be via repression of the production of intracellular c-di-GMP. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 08953988
- Volume :
- 35
- Issue :
- 9
- Database :
- Supplemental Index
- Journal :
- Biomedical & Environmental Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 159690803
- Full Text :
- https://doi.org/10.3967/bes2022.106