Transcriptome analysis of acute high temperature-responsive genes and pathways in Palaemon gravieri.

Temperature is an important variable factor in aquaculture which affects the health, survival, behavior, growth, and development of aquatic animals. Palaemon gravieri is one of the main economic shrimps in marine capture fisheries of the East China Sea and the South China Yellow Sea; however, it cannot tolerate high temperatures, thereby, resulting in unsuccessful large-scale farming. Thus far, there are few studies on the effects of acute high temperature on P. graviera. Therefore, it is especially important to study the effects of temperature fluctuations, especially acute high temperature, on P. gravieri. In this study, P. gravieri was treated with acute high-temperature stress, which gradually rose from 15 °C to 30 °C in 3 h, then remained at 30 °C for 12 h. The hepatopancreas of shrimps from five time points was collected once at 15 °C and thereafter, every 3 h after 30 °C. The samples of G0, G1, and G4 were selected for transcriptome analysis. A total of 18,308 unigenes were annotated, of which 7744 were differentially expressed. Most differentially expressed genes (DEGs) come from several physiological and biochemical processes, such as metabolism (GRHPR, ALDH5A1, GDH), immunity (HSP70, Rab5B, Rab10, CASP7), and stress-related process (UGT, GST, HSP60, HSP90). The results indicated that acute high temperature significantly reduced the metabolic capacity of shrimp but enhanced the immune capacity, which seemed to be an emergency metabolic compensation technique to resist stress. This study contributes to ongoing research on the physiological mechanism of P. gravieri response to acute high temperature. [Display omitted] • Transcriptome sequencing and analysis of P. gravieri was firstly performed. • DEGs of P. gravieri at different time points after high temperature stress were firstly compared. • The number of DEGs cumulative increased with prolonging the thermal treatment time. • The temporal expression patterns of DEGs were analyzed by STEM. • High temperature caused the inhibition of metabolism, while immune was enhanced. [ABSTRACT FROM AUTHOR]