STUDY OF GENETIC VARIATION IN KCI AND MgCI2 TREATED LOCAL BARLEY (Hordeum vulgare L.) WITH THE USE OF SSR AND RAPD MARKERS.

Genetic variation among the natural growing populations seems due to difficult ecological and environmental conditions that end in evolution. Molecular markers (RAPD & SSR) were applied to analysis ecological effects on the DNA of (Hordewn vulgare L.). This study was consisted with Hordiwn vulture genotype (Jow 83) and five levels of KCl and MgCl2 salt solution (0, 50, 100 and 150, 200 mM). Salt treatments inhibited growth parameters including germination 0%, rate of germination, fresh and dry weight. The genetic distances among the treated barley populations were analyzed by generating dendogram from RAPD and SSR fragments. Out of Five (5) RAPD primers produced a total of 219 loci in Horclizan vulgare genotype. Out of which 49 (9.4 %) were polymorphic band, whereas 172 (34.4%) were monomorphic band with an average (43.8%) generated both monomorphic and polymorphic. While two SSR primer produced a total 26 loci in barley genotypes, out of these 5 ( 2.5%) were polymorphic band, whereas 21 (9.5 %) were mon-omorphic band with an average (18.6%) generated both monomorphic and polymorphic. [ABSTRACT FROM AUTHOR]