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Antibacterial efficacy of silver nanoparticles against metallo-β-lactamase (blaNDM, blaVIM, blaOXA) producing clinically isolated Pseudomonas aeruginosa.

Authors :
Qureshi, Rabia
Qamar, Muhammad Usman
Shafique, Muhammad
Muzammil, Saima
Rasool, Muhammad Hidayat
Ahmad, Ijaz
Ejaz, Hasan
Source :
Pakistan Journal of Pharmaceutical Sciences; 2021 Supplement, Vol. 34, p237-243, 7p
Publication Year :
2021

Abstract

Carbapenem resistance in Pseudomonas aeruginosa is a major concern in the public health sector, primarily in developing countries such as Pakistan. Therefore, novel approaches such as Silver nanoparticles (AgNPs) can be used to address emerging concerns. Clinical isolates (n=200) were reconfirmed using selective media and API 20NE kit. The antibiogram was determined according to the CLSI 2016 guidelines. Molecular detection was carried out by PCR. Antibacterial activity in AgNPs was achieved by dilution method. Of 200 P. aeruginosa, mostly (n=82; 41%) were isolated from pus samples. Of 110 MDR P. aeruginosa, 70 (63%) were carbapenemase and 58 (52%) were MBL producers. Antimicrobial profile of MBL producing P. aeruginosa reported that all isolates were resistant to β-lactams, and 89% to levofloxacin and ciprofloxacin except colistin. Of 25 (35.7%) blaNDM producing P. aeruginosa, 12 isolates (48%) had MIC 16μg/mL to imipenem. Of 23 (32%) blaVIM producing P. aeruginosa, 12 (52%) contained MIC 16μg/mL to imipenem. However, 12 (17.1%) blaOXA-48 producing P. aeruginosa, 4 (33%) contained MIC 16μg/mL to imipenem. In vitro AgNPs activity inhibited and killed MBL producing isolates at 1 mg/mL and 2 mg/mL, respectively. AgNPs may be used as an alternative therapy followed by multiple clinical trials. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
1011601X
Volume :
34
Database :
Supplemental Index
Journal :
Pakistan Journal of Pharmaceutical Sciences
Publication Type :
Academic Journal
Accession number :
151223301
Full Text :
https://doi.org/10.36721/PJPS.2021.34.1.SUP.237-243.1